microinjector (Narishige inc)
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Microinjector, supplied by Narishige inc, used in various techniques. Bioz Stars score: 97/100, based on 709 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microinjector/Microinjector/pmc13068555-77-0-2
Average 97 stars, based on 709 article reviews
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Saline:Article Title: Reduced cortico-accumbal excitatory input due to Nav1.2 haploinsufficiency impairs sociability independently of dopamine Article Snippet: PV -Cre transgenic mice (>8 weeks of age, both sexes) were anesthetized with isoflurane (1.0–2.5%) and placed in a stereotaxic apparatus (Stoelting). .. Bilateral injections of AAV5-EF1α-DIO-hM4D(Gi)-mCherry (7.5 × 10 12 viral genomes/ml; 250 nl per site) or phosphate-buffered saline (PBS; 250 nl per site) into the NAc were performed using a Injection:Article Title: PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities Article Snippet: .. A single 400-nl injection at a rate of 5 nl s −1 was performed using a glass capillary and controlled by a Article Title: Noise-evoked dopamine dynamics in the nucleus accumbens and tail of the striatum Article Snippet: .. AAV9-CAG-dLight1.3b (100 nl; catalog # 125560-AAV9, Addgene, MA, USA) or AAV9-Syn-JRGECO1a (100 nl; catalog # 100854-AAV9, Addgene) was injected into the core of the NAc (in mm; AP: 1.2, ML: 1.4, from bregma; DV: -3.9 from the brain surface) or the TS (in mm; AP: -1.1, ML: 2.9, from bregma; DV: -2.8 from the brain surface) of C57BL/6J mice by using a Article Title: A method for CRISPR/Cas9-induced genetic barcoding and lineage tracing in sheep. Article Snippet: .. Cas9 mRNA (100 ng/μL), PB mRNA (100 ng/μL), sgRNAs (100 ng/μL), linearized plasmid libraries (10 ng/μL) and Rosa26 donor fragment (10 ng/μL) were mixed and injected using a Microinjection:Article Title: Efficient genome editing with chimeric oligonucleotide-directed editing. Article Snippet: Mouse Embryo Isolation and Culture Female mice (at least 6 weeks old) were superovulated with 5 IU pregnant mare serum gonadotropin (PMSG; Folligon, Merck Animal Health). .. The microinjection platform was equipped with a Inverted Microscopy:Article Title: Efficient genome editing with chimeric oligonucleotide-directed editing. Article Snippet: Mouse Embryo Isolation and Culture Female mice (at least 6 weeks old) were superovulated with 5 IU pregnant mare serum gonadotropin (PMSG; Folligon, Merck Animal Health). .. The microinjection platform was equipped with a Bioprocessing:Article Title: Defining a Midgestational Window for In Utero Genome Editing of the Fetal Murine Cortex Article Snippet: Glass capillary tubes (G100TF-4, OD 1.00 mm; Warner Instruments) were pulled on a vertical needle puller, cut using a microforge (MF2; Narishige) to a ∼20 μm tip diameter, and beveled at 20° using a microgrinder (EG-402; Narishige). .. Concentrated AAV or dextran dye (Thermo Fisher Scientific, D1817) was loaded into needles and mounted on a Plasmid Preparation:Article Title: A method for CRISPR/Cas9-induced genetic barcoding and lineage tracing in sheep. Article Snippet: .. Cas9 mRNA (100 ng/μL), PB mRNA (100 ng/μL), sgRNAs (100 ng/μL), linearized plasmid libraries (10 ng/μL) and Rosa26 donor fragment (10 ng/μL) were mixed and injected using a |
